C reactive protein (CRP) is an annular, pentameric protein found in human plasma whose levels rise in response to inflammation. It is synthesised by the liver in response to factors released by macrophages and fat cells. CRP plays a major role in activating the complement system, and is mainly used as a cardiac and inflammation marker. It is used as a positive control in CRP test kit. It is also used for the manufacturing of calibrators and controls in various in vitro diagnostic kits. Additionally, CRP is utilised for manufacturing protein purification columns for the antigen affinity purification of anti-CRP and as a coating antigen for ELISA or agglutination assays.
C Reactive Protein
Product Code : 00-AGN-AP-CRP-001
Specifications:
| Parameters | Acceptance Criteria |
|---|---|
| Physical Appearance | Clear Colourless Solution |
| CRP Concentration (Immunoturbidimetry method) |
1.3 to 3.0 mg/ml |
| CRP Concentration (OD at 280nm) | 1.3 to 3.0 mg/ml |
| Purity by SDS PAGE | > 99% Pure (Single band corresponding to Mol. Wt. of 23kDa) |
| Identity by Western Blot | React specifically with anti-CRP antibodies |
| Molecular Weight | 23 kDa |
| Form | Liquid |
| Storage buffer | Tris Saline with 15mM NaN3, pH 8.0 ± 0.2 |
| Storage temp | 2 to 8˚C |
| Shelf life | 3 years |
| Anti HIV-1 & 2 (ELISA) | Negative |
| Anti HCV (ELISA) | Negative |
| HBsAg Antigen (ELISA) | Negative |
| HBV DNA (PCR) | Not Detected |
| HCV RNA (PCR) | Not Detected |
| HIV DNA (PCR) | Not Detected |
| Source | Human pleural fluid and ascitic fluid |
For research use or further manufacturing purposes only